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IL 6 Mouse
英文名稱:IL 6 Mouse總訪問(wèn):259
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產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
規(guī)       格:CYT-350 最后更新:2024-12-5
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Catalogue Number CYT-350
Synonyms IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor.
Introduction Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
Description Interleukin-6 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.
The IL-6 is purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation The protein was lyophilized without any additives.
Solubility It is recommended to reconstitute the lyophilized Interleukin 6 in 10mM HCl to 0.1-1.0 mg/ml to regain full activity, and can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
Biological Activity The ED50 as determined by the dose-dependant induction of 7TD1 cell proliferation,  is 0.018 ng/ml corresponding to a Specific Activity of 55MIU/mg.
References Title:Interleukin-10 overexpression in macrophages suppresses atherosclerosis in hyperlipidemic mice.
Publication:Published online before print March 30, 2010, doi: 10.1096/fj.09-148155 August 2010 The FASEB Journal vol. 24 no. 8 2869-2880 .
Link:http://www.fasebj.org/content/24/8/2869.full
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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