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IL 27 Human
英文名稱:IL 27 Human總訪問(wèn):219
國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問(wèn):1
產(chǎn)地/品牌:prospec產(chǎn)品類別:細(xì)胞生物學(xué)試劑
規(guī)       格:CYT-048 最后更新:2024-12-5
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Catalogue Number CYT-048
Synonyms Interleukin-30, IL-30, IL27/p28, p28, Interleukin-27, Interleukin-27/p28, IL27, Interleukin-27 subunit alpha, IL27 subunit alpha, IL27-A, Il27, Il27a, IL27p28.
Introduction Interleukin-27 protein is related to IL-12A & is one of the subunits of a heterodimeric cytokine complex. IL27 interacts with EBV induced gene 3 also called EBI3, and forms a complex that drives rapid expansion of CD4 (+) T cells.
IL27 complex is synergizes with IL-12 in order to trigger the cytokine production of IFN-Gamma of CD4 (+) T cells. The biological effect of IL27 is mediated by class-I cytokine receptor (WSX1/TCRR).
The pro-inflammatory activity of IL27 is mediated through the growing expression of key molecules involved in the MHC class-I & MHC class-II pathways. Both MHC class-I and MHC-class-II expression are increased in endothelial cells after Interleukin-27 stimulation which suggests that it may play an important role in conferring the immune function on vascular endothelium IL27p28 subunit can be induced by IFN-beta and during LPS-induced maturation of dendritic cells in type-I IFN-dependent manner through IFN regulatory factor-1 activation.
Interleukin-27 regulates Interleukin-12 responsiveness of CD4+ T cells through Stat1-dependent and -independent mechanisms. IL17 & IL-23 play an important IL17 role in inflammation. Interleukin-27 possesses potent anti-angiogenic activity that plays an important role in its antitumor and antimetastatic activities.
EBV induced gene 3 plays a role, independently from IL27, in regulating anti-viral or anti-tumoral immune responses. Interleukin-27 is a potent inhibitor of HIV-1 replication in macrophages, CD4+ T cells, peripheral blood mononuclear cells.
Interleukin-27 triggers STAT activation and gene transcription.
Description Interleukin-27 Human Recombinant produced in HEK 293 is a glycosylated non-covalently linked heterodimer, consisting of one p28 subunit (215 amino acids) and one EBI3 subunit (209 amino acids), with a total a molecular weight of 47.8kDa.
Human IL27 is purified by proprietary chromatographic techniques.
Source HEK 293 cells
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from 10mM Na2PO4 + 50mM NaCl, pH 7.5.
Solubility It is recommended to reconstitute the lyophilized Interleukin-27 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Interleukin-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(c) UV spectroscopy at 280 nm.
Amino acid sequence

EBI3: RKGPPAALTL PRVQCRASRY PIAVDCSWTL PPAPNSTSPV SFIATYRLGM AARGHSWPCL QQTPTSTSCT ITDVQLFSMA PYVLNVTAVH PWGSSSSFVP FITEHIIKPD PPEGVRLSPL AERQLQVQWE PPGSWPFPEI FSLKYWIRYK RQGAARFHRV GPIEATSFIL RAVRRRARYY VQVAAQDLTD YGELSDWSLP ATATMSLGK

P28: FPRPPGRPQL SLQELRREFT VSLHLARKLL SEVRGQAHRF AESHLPGVNL YLLPLGEQLP DVSLTFQAWR RLSDPERLCF ISTTLQPFHA LLGGLGTQGR WTNMERMQLW AMRLDLRDLQ RHLRFQVLAA GFNLPEEEEE EEEEEEEERK GLLPGALGSA LQGPAQVSWP QLLSTYRLLH SLELVLSRAV RELLLLSKAG HSVWPLGFPT LSPQP
Biological Activity The activity is determined by its ability to inhibit IL-2 production from activated mouse spleenocytes and is typically 60-90ng/mL.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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