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IL 12 p40 Human, Plant
英文名稱(chēng):IL 12 p40 Human, Plant總訪問(wèn):237
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產(chǎn)地/品牌:prospec產(chǎn)品類(lèi)別:細(xì)胞生物學(xué)試劑
規(guī)       格:CYT-056 最后更新:2024-12-5
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Catalogue Number CYT-056
Synonyms NKSF2, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), TSF, Edodekin-alpha, IL-12 p40, IL-12B, IL-12 subunit p40, NK cell stimulatory factor chain 2.
Introduction Active IL-12 is a p70 disulphide-linked dimer composed of p35 and p40 subunits. The protein is a pleiotropic cytokine produced primarily by antigen presenting cells and has multiple effects on T lymphocytes and natural killer cells in terms of stimulating cytotoxicity, proliferation, production of other cytokines and Th1 subset differentiation.
Description IL-12 p40 human Recombinant produced in Nicotiana benthamiana plant is a glycosilated polypeptide chain containing 312 amino acids, 23-328 aa (molecular formula C1575H2420N424O490S12).
IL-12 p40 is fused to a 6-His-tag at the N-terminal having the total molecular mass of 35.5kDa however as a result of potential glycosylation, the molecular mass could be approximately 40kDa by SDS PAGE under reducing conditions.
IL-12 p40 IS purified by standard chromatographic techniques.
Source Nicotiana benthamiana plant
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7.4.
Solubility It is recommended to reconstitute the lyophilized IL-12 p40 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized IL-12 p40 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-12 p40 should be stored at 4°C between
2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 97.0% as determined by Analysis by SDS-PAGE.
Amino acid sequence HHHHHHIWEL KKDVYVVELD WYPDAPGEMV VLTCDTPEED GITWTLDQSS EVLGSGKTLT IQVKEFGDAG QYTCHKGGEV LSHSLLLLHK KEDGIWSTDI LKDQKEPKNK TFLRCEAKNY SGRFTCWWLT TISTDLTFSV KSSRGSSDPQ GVTCGAATLS AERVRGDNKE YEYSVECQED SACPAAEESL PIEVMVDAVH KLKYENYTSS FFIRDIIKPD PPKNLQLKPL KNSRQVEVSW EYPDTWSTPH SYFSLTFCVQ VQGKSKREKK DRVFTDKTSA TVICRKNASI SVRAQDRYYS SSWSEWASVP CS
Serological Identification The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific IL12 antibody.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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