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Catalogue Number | CYT-362 |
Synonyms | NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12. |
Introduction | IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. IL-12 is an initiator of cell-mediated immunity. |
Description | Interleukin-12 Human Recombinant produced in CHO is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. The IL-12 is purified by proprietary chromatographic techniques. |
Source | CHO (Chinese Hamster Ovarian Cells). |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | IL-12 was lyophilized from a 0.2?m filtered solution (0.5mg/ml) in PBS, pH 7.4. |
Solubility | It is recommended to reconstitute the lyophilized IL-12 in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized IL-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-12 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 98% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Amino acid sequence | The sequence of the first five N-terminal amino acids of IL-12 was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit. |
Biological Activity | IL-12 activity was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/ml corresponding to a specific activity of 2-3.3MU/mg. |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |