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Catalogue Number | CYT-500 |
Synonyms | B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor. |
Introduction | IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
Description | Interleukin-10 Human Recombinant produced in E.coli is a non covalent dimer of 2 polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton. The IL-10 is purified by proprietary chromatographic techniques. |
Source | Escherichia Coli. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution containing no additives. |
Solubility | It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability | Lyophilized Interleukin-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Amino acid sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. |
Biological Activity | The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be < 0.5ng/ml, corresponding to a Specific Activity of 2MIU/mg. |
References | Title:Activated signal transducer and activator of transcription-3 (STAT3) is a poor regulator of tumour necrosis factor- production by human monocytes. Publication:Article first published online: 9 JAN 2007 DOI:10.1111/j.1365-2249.2006.03291.x Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2006.03291.x/full |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |