Description:

Mouse monoclonal antibody

  Background:

HY5 is a basic leucine zipper (bZIP) transcription factor with nuclear localization. It is involved in light-regulated transcriptional activation of G-box-containing promoters. HY5 plays a role in anthocyanin accumulation in far-red light and blue light, but not in red light or in the dark.

  Synonyms:

HY5, ELONGATED HYPOCOTYL 5, REVERSAL OF THE DET PHENOTYPE 5, TED 5

  Immunogen:

Recombinant protein of HY5 derived from Arabidopsis thaliana AT5G11260.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Protein A purified

  Reconstitution:  

Reconstitution with 150µl of 0.01M sterile PBS. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

  Applications: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected Results: 

18 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

For more species homologues information, please contact tech support at tech@phytoab.com.

Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:1000 dilution overnight at 4℃.
Secondary antibody: 1:5000 dilution using Goat Anti-Mouse IgG H&L (HRP) (Cat# PHY6006)
Detection: using chemiluminescence substrate and image were captured with CCD camera.