![]() |
|
|||||||||||||||||||||||||||||||||
[發(fā)表評論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏] |
銷售商: 北京啟維益成科技有限公司 | 查看該公司所有產(chǎn)品 >> |
|
application information | ||
recommended dilution | 3 µg (WB) , 5 µg (IP for a 200 ul of a cell extract) | |
expected | apparent MW | 47.5 kDa | |
confirmed reactivity | Arabidopsis thaliana | |
predicted reactivity | please, inquire | |
not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
additional information | Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody. | |
selected references | to be added when available, antibody released in October 2013. |
5 µg of StrepTag-ABI1 protein extracted from Arabidopsis thaliana protoplasts with isolation buffer (20 mM Hepes, pH 7.5, 10 mM MgCl2, 1 mM DTT, 60 mM β-glycerophosphate, 1 mM NaF 1 mM phenylmethylsulfonyl fluoride, 0.1 mM NaVO3, Roche inhibitors)were separated on 12%SDS-PAGEand blotted 1h toPVDF. Blots were blocked with 3% semi-skimmed milk for 30 min. at room temperature (RT) with agitation. Blot was incubated with3 ug ofthe primary antibody for 30 minutes at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:50 000 in for 30min. at RT with agitation. The blot was washed as above and developed for 1 min with ECL according to the manufacturer's instructions.Exposure time was 5 min. Courtesy of Dr. Agnieszka Ludwików, Adam Mickiewicz University, Poland |
* 姓 名: |
* 地 區(qū): |
||
* 單 位: |
職 位: |
||
* 手機/電話: |
* E-mail: |
||
請寄產(chǎn)品資料: |
需要 不需要 | 請報價格: |
需要報價 不需要報價 |
留 言: |
|||
驗證碼: |
|
||