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                                V-PPase | vacuolar H+-pyrophosphatase
                                英文名稱:V-PPase | vacuolar H+-pyrophosphatase總訪問:554
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:3
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS121849 最后更新:2024-12-5
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                                product information
                                background

                                V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish proton gradient of often higher magnitude than H+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1, H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3

                                immunogen

                                KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,UniProt P31414, TAIR AT1G15690

                                antibody format

                                rabbit

                                polyclonal,

                                serum,

                                lyophilized

                                quantity

                                100 µl

                                storage

                                store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                collection of antibodies to tonoplast proteins

                                AS09 492 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

                                AS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)

                                AS09 494 | anti-TIP2;1 (delta) | Delta-VM23

                                AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1

                                AS09 510 | anti-TIP2;2 | tonoplast intrinistic protein 2-2

                                AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)

                                additional information Antibodies will detect target protein in 1 µg of a crude membrane preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, less than µg load per well should be sufficient.

                                Protocol of isolation of vacuolar membrane is available here.

                                application information
                                recommended dilution

                                1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IL)

                                expected | apparent MW

                                80.8 | 73 kDa (Arabidopsis thaliana)

                                confirmed reactivity

                                Arabidopsis thaliana, Picea abies

                                predicted reactivity

                                dicots including: Nicotiana tabacum, Ricinus communis, Vitis vinifera, Saccharum officinarum, monocots including: Horderum vulgare, Oryza sativa, Zea mays, trees: Populus trichocarpa

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

                                selected references

                                to be added when available, antibody release in March 2012.


                                application example


                                western blot using anti-VPP-ase antibodies


                                Different amounts (depending of the tissue) of membrane proteins from developing xylem tissue of Norway spruce were separated in a gradient (4-15 %) SDS-PAGE gel and blotted 30 min to nitrocellulose membrane using a standard semi-dry Trans-Blot ® Turbo ™ (Bio-Rad) system. Blots were blocked with 5% non-fat milk protein for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody V-PPase (Agrisera) at a dilution of 1: 5 000 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly, and then washed 3 times for 15 min in TBS-T at RT with agitation. Blot was incubated in the secondary antibody (anti-rabbit IgG horseradish peroxidase conjugate, from Agrisera, AS09 602) diluted to 1: 10 000 for 1 h at RT with agitation. The blot was washed as above and the presence of V-PPase detected with ECL according to the manufacturer's instructions. Exposure time was ~ 1 second.

                                Courtesy of Luis Alexis Jimenez Barboza
                                bio-equip.com
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