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                                ZCP2 | Zinc Chaperone Protein
                                英文名稱:ZCP2 | Zinc Chaperone Protein總訪問:321
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                                規(guī)       格:AS121848 最后更新:2024-12-5
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                                product information
                                background

                                COG0523 (Zinc defficiency induced protein) is a putative metal chaperone from G3E family of GTPases which acts in metal trafficking.

                                immunogen

                                recombinant ZCP2 protein, ID 536252

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                50µl

                                for reconstitution add 50 µl, of sterile water.

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products collection of antibodies to various Chlamydomonas proteins
                                additional information
                                application information
                                recommended dilution

                                1 : 1000 with ALP (WB)

                                expected | apparent MW

                                70 | 100 kDa (probably due to glycosylation)

                                confirmed reactivity

                                Chlamydomonas reinhardtii

                                predicted reactivity Chlamydomonas reinhardtii
                                not reactive in

                                no confirmed exceptions from predicted reactivity are currently known

                                additional information

                                This antibody can be used as a marker of zinc deficiency in Chlamydomonas.

                                selected references

                                to be added when available, antibody release in May 2012


                                application example

                                western blot using anti-COG0523 Zinc deficiency protein

                                Chlamydomonas reinhardtii soluble proteins (10 µg) were separated on a 7.5% SDS-PAGE gel and blotted to nitrocellulose for 90 min. at 1.5 mA cm-2. The membrane was blocked with 1% milk in PBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 2 hr at RT with agitation. The antibody solution was
                                decanted and the blot was rinsed briefly twice, then washed 3 times for 5 min in PBS-T + 1% milk at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse alkaline phosphatase conjugated, from Southern Biotech ) diluted to 1:3000 in PBS-T + 1% milk for 45 min at RT with agitation. The membrane was washed 2 times for 5 min in PBS-T + 1% milk at RT with agitation, then rinsed with TBS (pH 7.5), and developed.

                                Courtesy Dr. Dudley Page, UCLA, USA

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