| product information
| | background | | AAA2 domain is present in ATP binding proteins involved in stress response and being members of clpA/clpB family. | | immunogen | | KLH-conjugated synthetic peptide derived from proteins containing AAA2 domain, including Arabidopsis thaliana ClpB1 P42730, At1g74310 | | antibody format | | rabbit | polyclonal | | serum | lyophilized | | | quantity | | 200 µl | for reconstitution add 200 µl, of sterile water. | | | storage | | store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. | | tested applications | | western blot (WB) | | related products | | antibodies to other ATPases | | additional information | | to be added when available | |
application information
|
| recommended dilution | | 1 : 2000 with standard ECL (WB) |
| expected | apparent MW | | kDa |
| confirmed reactivity | | Arabidopsis thaliana, Synechococcus sp. PCC 7942
|
| predicted reactivity | | AAA2 domain containing proteins including S. cerevisiae HSP104,
|
| not reactive in | | no confirmed exceptions from predicted reactivity are currently known |
| additional information | | to be added when available |
| selected references | | to be added when available, antibody released in October 2011. |
application example
0.3 µg of total protein from Synechococcus sp. PCC7942 (1) and 20 µg of leaf soluble proteins from 3-week old Arabidopsis thaliana leaves (2) were separated on 3-8 % Tris-acetate NuPage gel (invitrogen) and blotted 1,5 h to supported introcellulose. Blots were blocked for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in 1xTBS-T for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:75 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 120 seconds.
Courtesy of Dr. A. Clarke, Göteborg University, Sweden
bio-equip.com
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