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                                FH14 | Formin-like protein 14 (50 ug)
                                英文名稱:FH14 | Formin-like protein 14 (50 ug)總訪問:465
                                國產(chǎn)/進口:進口半年訪問:3
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS111750 最后更新:2024-12-5
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                                product information
                                background

                                Formins are known to regulate microfilaments and associate with microtubles. Formin 14 acts as a linking protein between microtubules and microfilaments and plays an important role in the process of plant cell division.

                                immunogen

                                recombinant formin of Arabidopsis thaliana, amino acids 340-520, NP_17446

                                antibody format

                                mouse

                                monoclonal

                                IgG1

                                liquid

                                quantity

                                50 µg

                                storage

                                store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Antibodyis stable one year from the date of shipment.

                                tested applications

                                western blot (WB), immunofluorescence (IF)

                                related products

                                AS11 1752 | Anti-AtFH14 | Formin-like protein 14 (100 ug), mouse antibody

                                AS10 702 | Anti-actin-11 mouse antibody

                                AS10 681 | Anti-tubulin beta chain rabbit antibody

                                AS10 680 | Anti-tubulin alpha chain rabbit antibody


                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1 : 1000 - 1: 2000 with standard ECL (WB), 1:100 - 1: 500 (IF)

                                expected | apparent MW

                                115 kDa

                                confirmed reactivity

                                Arabidopsis thaliana

                                predicted reactivity Arabidopsis thaliana
                                not reactive in

                                no confirmed exceptions from predicted reactivity are currently known

                                additional information

                                to be added when available

                                selected references

                                Li et al. (2010). The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division. Plant Cell (8):2710-2726.


                                application example

                                immunofluorescence detection using anti-formin antibodies

                                Arabidopsis thaliana suspension cells were fixed in 4% (w/v) paraformaldehyde in PEM buffer (50 mM PIPES, 5 mM EGTA, 5 mM MgSO4, 0.1 M mannitol, pH 6.9) for 30 min at room temperature. Wash has been done with with PEM buffer and treated with 0.5% pectolyase and 1% cellulase R-10 in PEM buffer for 10 min at RT. Blocking was done in (w/v) BSA in PEM buffer. Incubation with primary antibodies at a dilution of 1: 100 has been done overnight at 48°C in a dark and moist chamber.
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