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                                                              FH14 | Formin-like protein 14 (50 ug)
                                                              英文名稱:FH14 | Formin-like protein 14 (50 ug)總訪問:465
                                                              國產(chǎn)/進口:進口半年訪問:3
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS111750 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              Formins are known to regulate microfilaments and associate with microtubles. Formin 14 acts as a linking protein between microtubules and microfilaments and plays an important role in the process of plant cell division.

                                                              immunogen

                                                              recombinant formin of Arabidopsis thaliana, amino acids 340-520, NP_17446

                                                              antibody format

                                                              mouse

                                                              monoclonal

                                                              IgG1

                                                              liquid

                                                              quantity

                                                              50 µg

                                                              storage

                                                              store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Antibodyis stable one year from the date of shipment.

                                                              tested applications

                                                              western blot (WB), immunofluorescence (IF)

                                                              related products

                                                              AS11 1752 | Anti-AtFH14 | Formin-like protein 14 (100 ug), mouse antibody

                                                              AS10 702 | Anti-actin-11 mouse antibody

                                                              AS10 681 | Anti-tubulin beta chain rabbit antibody

                                                              AS10 680 | Anti-tubulin alpha chain rabbit antibody


                                                              additional information

                                                              to be added when available

                                                              application information
                                                              recommended dilution

                                                              1 : 1000 - 1: 2000 with standard ECL (WB), 1:100 - 1: 500 (IF)

                                                              expected | apparent MW

                                                              115 kDa

                                                              confirmed reactivity

                                                              Arabidopsis thaliana

                                                              predicted reactivity Arabidopsis thaliana
                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity are currently known

                                                              additional information

                                                              to be added when available

                                                              selected references

                                                              Li et al. (2010). The type II Arabidopsis formin14 interacts with microtubules and microfilaments to regulate cell division. Plant Cell (8):2710-2726.


                                                              application example

                                                              immunofluorescence detection using anti-formin antibodies

                                                              Arabidopsis thaliana suspension cells were fixed in 4% (w/v) paraformaldehyde in PEM buffer (50 mM PIPES, 5 mM EGTA, 5 mM MgSO4, 0.1 M mannitol, pH 6.9) for 30 min at room temperature. Wash has been done with with PEM buffer and treated with 0.5% pectolyase and 1% cellulase R-10 in PEM buffer for 10 min at RT. Blocking was done in (w/v) BSA in PEM buffer. Incubation with primary antibodies at a dilution of 1: 100 has been done overnight at 48°C in a dark and moist chamber.
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