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                                Cyt c6 | thylakoid lumen cytchrome c6 protein
                                英文名稱:Cyt c6 | thylakoid lumen cytchrome c6 protein總訪問:211
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                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS06202 最后更新:2024-12-5
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                                product information
                                background

                                Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
                                Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis..
                                Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.

                                immunogen

                                GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                200 µl

                                for reconstitution add 200 µl of sterile water.

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS06 122 | anti-CRD1 | cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranes


                                AS05 084 | anti-PsbA rabbit antibody, marker of thylakoid membrane


                                Collection of antibodies to Chlamydomonas proteins

                                additional information

                                cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii

                                application information
                                recommended dilution

                                1 : 1 000 with alkaline phosphatase (WB)

                                expected | apparent MW

                                15 kDa

                                confirmed reactivity

                                Chlamydomonas reinhardtii

                                predicted reactivity

                                Chlorella vulgaris, Pediastrum duplex, Cladophora glomerata

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below

                                selected references

                                Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.


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