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                                Fer2 | ferrritin 2
                                英文名稱:Fer2 | ferrritin 2總訪問:423
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:2
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS06196 最后更新:2024-12-5
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                                product information
                                background

                                Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.

                                immunogen

                                KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii

                                antibody format

                                rabbit,

                                polyclonal

                                serum,

                                lyophilized

                                quantity

                                200 µl

                                - for reconstitution add 200 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS06 126 Fer1 | ferritin 1(pre-apoferritin)

                                Collection of antibodies to Chlamydomonas proteins

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1 : 1000

                                expected | apparent MW

                                32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii

                                confirmed reactivity

                                Chlamydomonas reinhardtii

                                predicted reactivity

                                Chlamydomonas reinhardtii

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                a picture with a western blot application example can be found in the reference given below

                                selected references

                                Long et al. (2008) Genetics 179: 137-147


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