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                                                              Gamma-ECS | gamma glutamylcysteine synthase
                                                              英文名稱:Gamma-ECS | gamma glutamylcysteine synthase總訪問:355
                                                              國產(chǎn)/進口:進口半年訪問:1
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS06186 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

                                                              immunogen

                                                              KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              total IgG in PBS pH 7.4

                                                              lyophilized

                                                              quantity

                                                              100 µl

                                                              for reconstitution add 100 µl of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB), immunolocalization (IL)

                                                              related products

                                                              collection of antibodies to plant stress proteins

                                                              additional information

                                                              total IgG concentration is 5.2 µg/ µl

                                                              application information
                                                              recommended dilution

                                                              1: 5000 with standard ECL (WB), 1: 5000 (IL)

                                                              expected | apparent MW

                                                              50 kDa

                                                              confirmed reactivity

                                                              Arabidopsis thaliana, Nicotiana tabcum, Solanum lycopersicum, Zea mays

                                                              predicted reactivity

                                                              dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information

                                                              antibodies has been used in immunolocalization on Arabidopsis thaliana

                                                              selected references Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

                                                              Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.


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