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                                Gamma-ECS | gamma glutamylcysteine synthase
                                英文名稱:Gamma-ECS | gamma glutamylcysteine synthase總訪問:355
                                國產(chǎn)/進口:進口半年訪問:1
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS06186 最后更新:2024-12-5
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                                product information
                                background

                                Gamma-glutamylcysteine synthase (gamma-ECS) (EC 6.3.2.2) catalyses the first step of glutathione (GSH) synthesis, producing gamma-glutamylcysteine (gamma-EC) from L-glutamate and cysteine. In the second step, catalyzed by GSH-S, glycine is added to the C-terminal end of (gamma-EC). GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development.

                                immunogen

                                KLH-conjugated synthetic peptide derived from Zea mays gamma-ECS Q8W4W3

                                antibody format

                                rabbit

                                polyclonal

                                total IgG in PBS pH 7.4

                                lyophilized

                                quantity

                                100 µl

                                for reconstitution add 100 µl of sterile water.

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB), immunolocalization (IL)

                                related products

                                collection of antibodies to plant stress proteins

                                additional information

                                total IgG concentration is 5.2 µg/ µl

                                application information
                                recommended dilution

                                1: 5000 with standard ECL (WB), 1: 5000 (IL)

                                expected | apparent MW

                                50 kDa

                                confirmed reactivity

                                Arabidopsis thaliana, Nicotiana tabcum, Solanum lycopersicum, Zea mays

                                predicted reactivity

                                dicots including:Pisum sativum, Riciuns communis, monocots including: Oryza sativa, Triticum aestivum

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                antibodies has been used in immunolocalization on Arabidopsis thaliana

                                selected references Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

                                Ghanta et al. (2011). Nicotiana tabacum overexpressing γ-ECS exhibits biotic stress tolerance likely through NPR1-dependent salicylic acid-mediated pathway. Planta 233(5):895-910.


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