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                                SPS | sucrose phosphate synthase, maize
                                英文名稱:SPS | sucrose phosphate synthase, maize總訪問:248
                                國產(chǎn)/進口:進口半年訪問:2
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS06185 最后更新:2024-12-5
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                                product information
                                background

                                SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

                                immunogen

                                Synthetic peptide derived from Zea mays SPS protein sequence (P31927).

                                antibody format

                                rabbit

                                polyclonal

                                total IgG in PBS pH 7.4

                                lyophilized

                                quantity

                                100 µl

                                - for reconstitution add 100 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS03 035A SPS | sucrose phosphate synthase, global

                                for other antibodies to various maize proteins, please inquire.

                                additional information

                                total IgG concentration is 3 µg/µl

                                application information
                                recommended dilution

                                1 : 2 000 with standard ECL (WB)

                                expected | apparent MW

                                120 | ~130 for Zea mays

                                confirmed reactivity

                                Zea mays

                                predicted reactivity

                                monocots:Saccharum officinarum, Triticum aestivum and Oryza sativa

                                not reactive in

                                Hordeum vulgare

                                additional information

                                to be added when available

                                selected references

                                Sparks et al. (2001) A Appl Biol 138: 33-45.Potential for manipulating carbon metabolism in wheat


                                application example

                                10 µg of total leaf protein from (1) A.thaliana, (3) Zea mays and (4) Hordeum vulgare extracted with PEB (AS08 300) as well as 10 µg cytosolic protein from (2) A.thaliana were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1.5h (30V) to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-SPS (AS06 0185, 1:2000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations where followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, high sensitivity).

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