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                                                              AO | apoplastic ascorbate oxidase
                                                              英文名稱:AO | apoplastic ascorbate oxidase總訪問:291
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS06184 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

                                                              immunogen

                                                              ascorbate oxidase purified from Cucurbita sp.

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              total IgG in PBS pH 7.4

                                                              lyophilized

                                                              quantity

                                                              100 µl

                                                              for reconstitution add 100 µl of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB)

                                                              related products

                                                              AS09 383 | anti-ascorbate oxidase biotinylated antibody

                                                              additional information

                                                              IgG concentration is 4 µg/µl

                                                              application information
                                                              recommended dilution

                                                              1:2 000 with standard ECL (WB)

                                                              expected | apparent MW

                                                              61 | 45 kDa (Cucurbita pepo)

                                                              confirmed reactivity

                                                              Cucurbita sp., Nicotiana tabacum, Solanum tuberosum, Zea mays

                                                              predicted reactivity

                                                              dicots including Glycine max, Cucumis melo, monocots including Oryza sativa

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information

                                                              antibody can be used as a marker of apoplast

                                                              selected references

                                                              Kielbowicz et al. (2008) The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability. J. Expo Bot. 59:2191-2203.


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