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                                                              cAPX | cytosolic ascorbate peroxidase
                                                              英文名稱:cAPX | cytosolic ascorbate peroxidase總訪問:390
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:1
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS06180 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              Ascorbate peroxidase (APX) is the enzyme catalyzing the ascorbate-dependent reduction of hydrogen peroxide. Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in fruits and photosynthetic tissues. AA is found in every compartment of the plant cell including the apoplast.

                                                              immunogen

                                                              KLH-conjugated peptide derived from N-terminal of Zea mays cytosolic APX Q41772

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              total IgG in PBS pH 7.4

                                                              lyophilized

                                                              quantity

                                                              200 µl

                                                              for reconstitution add 200 µl of sterile water.

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB), immunoprecipitation (IP), immunolocalization (IL)

                                                              related products

                                                              AS06 184 | anti-AO | apoplastic ascorbate oxidase

                                                              AS09 384 | anti-AO | ascorbate oxidase

                                                              AS09 383 | anti-AO | ascorbate oxidase, biotinylated antibody

                                                              AS08 368 | anti-APX | ascorbate peroxidase

                                                              additional information

                                                              total IgG concentration is 3 µg/µl

                                                              application information
                                                              recommended dilution

                                                              1:10 000 with standard ECL (WB), 2 µg (IP), 1: 500 (IL)

                                                              expected | apparent MW

                                                              28 kDa

                                                              confirmed reactivity

                                                              Arabidopsis thaliana (immunohistochemistry only) D. salina, A. toxicaria, Horderum vulgare, Pisum sativum, Silene vulgaris, S. lycopersicum, , Solanum tuberosum, P. silvestris, Zea mays

                                                              predicted reactivity

                                                              dicots including: Brassica. juncea, Fragaria ananassa, Gossypium hirsutum, Nicotiana tabacum,, Solanum tuberosum, Vitis vinifera, monocots including: Oryza sativa, trees: Pinus pinaster

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information
                                                              selected references Tsaniklidis et al. (2013). L-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Regul,DOI 10.1007/s10725-013-9845-0. (Solanum lycopersicum, immunolocalization)

                                                              Correa-Aragunde et al. (2013). Auxin induces redox regulation of ascorbate peroxidase 1 activity by S-nitrosylation/denitrosylation balance resulting in changes of root growth pattern in Arabidopsis. J Exp Bot, Aug;64(11):3339-49.

                                                              Wu et al. (2013). Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus. J. Proteomics, June 14th.

                                                              Sobrino-Plata et al. (2013). Specific stress responses to cadmium, arsenic and mercury appear in the
                                                              2 metallophyte Silene vulgaris when grown hydroponically. RSC Advances, Jan 24.

                                                              Bai et al. (2011). Nitric Oxide Enhances Desiccation Tolerance of Recalcitrant Antiaris toxicaria Seeds via Protein S-Nitrosylation and Carbonylation. PLoSONE.

                                                              application example

                                                              (A) , (B)- control antibody, anti-PIN1, (C,D) immunolocalization using anti-cAPX antibodies.

                                                              Courtesy of Dr. Taras Pasternak


                                                              immunolocalization using anti-cAPX antibodies
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