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                                CDJ1 | chloroplast DnaJ homolog 1
                                英文名稱:CDJ1 | chloroplast DnaJ homolog 1總訪問:284
                                國產(chǎn)/進口:進口半年訪問:1
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS06179 最后更新:2024-12-5
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                                product information
                                background

                                CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

                                immunogen

                                fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                100 µl

                                for reconstitution add 100 µl of sterile water.

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB) immunoprecipitation (IP)

                                related products

                                collection of antibodies to other heat shock proteins

                                collection of antibodies to Chlamydomonas reinhardtii

                                collection of antibodies to chloroplast proteins

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1. 3000with standard ECL (WB)

                                expected | apparent MW

                                40 | 40 kDa

                                confirmed reactivity

                                Chlamydomonas reinhardtii

                                predicted reactivity

                                Ostreococcus sp.

                                not reactive in

                                higher plants

                                additional information

                                western blot has been done on 10-50 µg load per well; immunoprecipitation has been done on 5-50 µl

                                selected references

                                Willmund et al (2008).The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C.Plant Physiol. 148: 2070-2082.


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