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                                8-Hydroxyguanosine | DNA/RNA oxidative damage (25 μg)
                                英文名稱:8-Hydroxyguanosine | DNA/RNA oxidative damage (25 μg)總訪問:374
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                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS10708-25 最后更新:2024-12-5
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                                product information
                                background

                                Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.

                                immunogen

                                8-hydroxy-guanosine-BSA and – casein conjugates

                                antibody format

                                mouse

                                monoclonal

                                IgG2A (clone 15A3)

                                liquid

                                quantity

                                25 µg

                                storage

                                store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                ELISA (ELISA), immunostaining on frozen tissue and paraffin-embedded (IHC-Fr-P), affinity purification (AP)

                                related products

                                AS10 708-100 | hydroxy guanosine monoclonal antibody, 100 µg

                                additional information

                                protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml

                                application information
                                recommended dilution

                                needs to be determined for each application

                                expected | apparent MW
                                confirmed reactivity

                                Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)

                                predicted reactivity
                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                protocol for immunostaining using this antibody can be found here

                                selected references

                                Pilger & Rudiger (2006). 8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures. Int Arch Occup Environ Health. 1: 1-15.

                                Kowluru et al. (2006). Role of mitochondrial superoxide dismutase in the development of diabetic retinopathy.Invest Ophthalmol Vis Sci 4: 1594-9.

                                Kim et al. (2003). Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells. Carcinogenesis 2: 235-241.

                                Cui et al. (2000).Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain. Faseb J. 14(7): 955-67.


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