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                                                              3-nitroY | nitrotyrosine (25 μl)
                                                              英文名稱:3-nitroY | nitrotyrosine (25 μl)總訪問:272
                                                              國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問:4
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS10706-25 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species.

                                                              immunogen

                                                              BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl
                                                              acetamido) propionic acid

                                                              antibody format

                                                              mouse

                                                              monoclonal

                                                              IgG2A

                                                              liquid

                                                              quantity

                                                              25 µl

                                                              storage

                                                              store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB), ELISA (ELISA), immunohistochemistry (IHC), immunoprecipitation (IP)

                                                              related products

                                                              AS10 706-100 | anti-nitrotyrosine mouse antibody, 100 µg

                                                              additional information

                                                              1 mg/ml of Protein G purified IgG2A in PBS pH 7.4, 0.09 % sodium azide, 50 % glycerol

                                                              application information
                                                              recommended dilution

                                                              needs to be determined for each application

                                                              expected | apparent MW

                                                              n.a.

                                                              confirmed reactivity

                                                              human, mouse, rat, dog

                                                              predicted reactivity

                                                              higher plants

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information

                                                              antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity
                                                              with non-nitrated tyrosine. Not species specific.

                                                              0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL

                                                              selected references

                                                              Antibody used in western blot and ELISA: Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387. Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.

                                                              Antibody used in immunohistochemistry: Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.


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