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                                HemH | protoporphyrin ferrochelatase (cyanobacterial)
                                英文名稱(chēng):HemH | protoporphyrin ferrochelatase (cyanobacterial)總訪問(wèn):268
                                國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問(wèn):1
                                產(chǎn)地/品牌:agrisera產(chǎn)品類(lèi)別:植物試劑
                                規(guī)       格:AS10693 最后更新:2024-12-5
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                                product information
                                background

                                HemH is ptotoporphyrin ferrochelatase of heme biosynthesis.

                                immunogen

                                KLH-conjugated peptide derived from cyanobacterial ferrochelatase protein sequence

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                100 µl

                                for reconstitution add100 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS07 228 | HemH | protoporphyrin ferrochelatase (reacts with higher plants)

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1: 3000 with standard ECL, West pico (WB)

                                expected | apparent MW

                                46 kDa

                                confirmed reactivity

                                Synechocystis sp. PCC 6803

                                predicted reactivity

                                cyanobacteria

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                loads higher than 0.5 µg of chlorophyll per well are not recommended

                                selected references

                                to be added when available


                                application example

                                Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

                                Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon,
                                Czech Republic




                                western blot using anti-ferrochelatase antibodies (cyanobacterial)

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