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                                當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > PsbI | PsbI | small subunit I of PSII (cyanobacterial)
                                PsbI | PsbI | small subunit I of PSII (cyanobacterial)
                                英文名稱:PsbI | PsbI | small subunit I of PSII (cyanobacterial)總訪問(wèn):246
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                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS10692 最后更新:2024-12-5
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                                product information
                                background

                                The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein

                                immunogen

                                KLH-conjugated synthetic peptide derived from Synechocystis sp. PCC 6803 PsbI sequence Q54697

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                200 µl

                                for reconstitution add 200 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS06 158 | PsbI | small subunit I of PSII

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1: 5000 - 1: 10 000 with standard ECL West pico (WB)

                                expected | apparent MW

                                4.3 kDa

                                confirmed reactivity

                                Synechocystis sp. PCC 6803

                                predicted reactivity

                                cyanobacteria

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                loads higher than 0.5 µg of chlorophyll per well are not recommended

                                selected references

                                Dobakova et. al (2007). Role of the PsbI Protein in
                                Photosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.


                                application example

                                Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

                                Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon,
                                Czech Republic





                                western blot detection using anti-PsbI antibodies (cyanobacterial)
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