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                                                              AO | ascorbate oxidase
                                                              英文名稱:AO | ascorbate oxidase總訪問:548
                                                              國產(chǎn)/進口:進口半年訪問:10
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS09384 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

                                                              immunogen

                                                              ascorbate oxidase purified from Cucurbita sp.

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              total IgG in PBS pH 7.2

                                                              lyophilized

                                                              quantity

                                                              1 mg

                                                              for reconstitution add 0.5 ml of sterile water

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

                                                              tested applications

                                                              Western blot (WB), ELISA(ELISA), immunohistochemistry (IHC)

                                                              related products

                                                              AS06 184 | anti-ascorbate oxidase rabbit antibody

                                                              AS09 383 | anti-ascrobate oxidase biotinylated rabbit antibody

                                                              additional information

                                                              IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography.

                                                              application information
                                                              recommended dilution

                                                              1: 1000 (WB), 1: 1000 - 1: 30 000 (ELISA)

                                                              expected | apparent MW

                                                              61 | 45 kDa (Cucurbita pepo)

                                                              confirmed reactivity

                                                              Cucurbita sp.

                                                              predicted reactivity

                                                              dicots including Glycine max, monocots including Oryza sativa

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information

                                                              to be added when available

                                                              selected references

                                                              to be added when available


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