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GlnA | glutamine synthetase protein standard

英文名稱：	GlnA \| glutamine synthetase protein standard	總訪問：	225
國產(chǎn)/進(jìn)口：	進(jìn)口	半年訪問：	11
產(chǎn)地/品牌：	agrisera	產(chǎn)品類別：	植物試劑
規(guī) 格：	AS09018S	最后更新：	2024-12-5
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銷售商：北京啟維益成科技有限公司

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product information

background

Glutamine synthetase (GlnA) is the key enzyme in the incorporation of mineral nitrogen into glutamine.

This product is a recombinant GlnA protein standard, source Synechocystis strain PCC 6803.

immunogen

antibody format

does not apply

quantity

250 µl

lyophilized, for reconstitution add 225 µl of milliQ water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB)

related products

collection of other protein standards

AS01 018 | anti-GlnA | glutamine synthetase hen antibody

collection of other global antibodies

collection of antibodies to proteins involved in nitrogen metabolism

additional information

The GlnA protein standard can be used in combination with global anti-GlnA antibodies to quantitate GlnA from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the GlnA protein.

Quantitative western blot: detailed method description, video tutorial

application information
recommended dilution		Standard curve: 3 loads are recommended (0.5, 2 and 4μl). For most applications a sample load of 0.2μg of chlorophyll will give a GlnA signal in this range. Positive control: a 2μl load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.
expected \| apparent MW		in most gel systems GlnA migrates around 53 kDa
confirmed reactivity
predicted reactivity
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.20 pmol/µl Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT. This standard is ready-to-load and does not require any additions or heating.
selected references		to be added when available

application example

3 µg of total protein from Trichodesmium IMS 101 extracted with PEB (AS08 300) (1-8) and GlnA protein standard 0.3, 0.15, 0.07 pmol (9-11) were separated on 4-12% NuPage(Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-hen IgY horseradish peroxidase conjugated, from Abcam) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance dete

北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領(lǐng)域多年銷售經(jīng)驗(yàn)人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應(yīng)商建立了長期穩(wěn)定的合作關(guān)系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內(nèi)客戶的最新需求，我們也與時(shí)俱進(jìn)地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標(biāo)是把國外有特點(diǎn)的生物前沿產(chǎn)品引進(jìn)中國，讓國內(nèi)科學(xué)研究機(jī)構(gòu)和制藥企業(yè)盡早使用上最新型的科研產(chǎn)品。

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