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                                HemC | porphobilinogen-Deaminase (PBG)
                                英文名稱:HemC | porphobilinogen-Deaminase (PBG)總訪問:304
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:4
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS08375 最后更新:2024-12-5
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                                product information
                                background

                                PBG is an enzyme involved in tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

                                immunogen

                                overexpressed Thermosynechococcus elongatus HemC Q8DIE4

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                200 µl

                                for reconstitution add 200 µl of sterile water.

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS07 226 | anti-HemF

                                AS07 228 | anti-HemH | protoporphyrin ferrochelatase

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1: 1000 - 1: 5000 (WB)

                                expected | apparent MW

                                34.7 kDa

                                confirmed reactivity

                                Thermosynechococcus elongatus

                                predicted reactivity

                                cyanobacteria

                                not reactive in

                                Synechococcus sp. PCC 6803

                                additional information

                                to be added when available

                                selected references

                                to be added when available


                                application example

                                0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

                                Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon

                                wesern blot detection using anti-PBG (HemC) antibodies
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