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application information | ||
recommended dilution | 1: 1000 - 1: 5000 (WB) | |
expected | apparent MW | 34.7 kDa | |
confirmed reactivity | Thermosynechococcus elongatus | |
predicted reactivity | cyanobacteria | |
not reactive in | Synechococcus sp. PCC 6803 | |
additional information | to be added when available | |
selected references | to be added when available |
application example 0.5 ug chl from Synechocystissp. PCC 6803 membrane fraction (1) and soluble fraction (2) and Thermosynechococcus elongatus (3); Synechocystis was prepared as a soluble and membrane fractions. Separated on 12% SDS-PAGE and blotted overnight at 4C to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS. Probed with anti-HemC antibody in TTBS (AS08 357, 1:2000, 4h) and secondary anti-rabbit in TTBS (1:10000,1h). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000. Courtesy Dr. Roman Sobotka Laboratory of Photosynthesis, Trebon | ![]() |