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                                當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > Tlp18.3 | Thylakoid lumen 18.3 kDa protein
                                Tlp18.3 | Thylakoid lumen 18.3 kDa protein
                                英文名稱:Tlp18.3 | Thylakoid lumen 18.3 kDa protein總訪問:648
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:11
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS08369 最后更新:2024-12-5
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                                product information
                                background

                                UPF0603 protein At1g54780, chloroplastic is located in thylakoid lumen. The protein seem to be required for high light tolerance of PSII. Sirpiö et al. (2007) TLP18.3, a novel thylakoid lumen protein regulating photosystem II repair cycle. Biochem J; 406(3): 415-425

                                immunogen

                                synthetic peptides amino acids 227-241 and amino acids 242-256 from Arabidopsis thaliana Tlp18.3 protein sequence (accession At1g54780)

                                antibody format

                                rabbit;

                                polyclonal;

                                serum;

                                lyophilized

                                quantity

                                200 µl

                                - for reconstitution add 200 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                Western blot (WB)

                                related products

                                to be added when available

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1: 1000 with standard ECL (WB)

                                expected | apparent MW

                                22 | 18.5 kDa for A. thaliana

                                confirmed reactivity

                                Arabidopsis thaliana

                                predicted reactivity

                                Oryza sativa, Ricinus communis, Zea mays

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                application note: in a gel without urea Tlp18.3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa

                                selected references

                                Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16.


                                application example

                                                                                4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL.
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