![]() |
|
|||||||||||||||||||||||||||||||||
[發(fā)表評論] [本類其他產(chǎn)品] [本類其他供應(yīng)商] [收藏] |
銷售商: 北京啟維益成科技有限公司 | 查看該公司所有產(chǎn)品 >> |
|
application information | ||
recommended dilution | 1: 1000 with standard ECL (WB) | |
expected | apparent MW | 22 | 18.5 kDa for A. thaliana | |
confirmed reactivity | Arabidopsis thaliana | |
predicted reactivity | Oryza sativa, Ricinus communis, Zea mays | |
not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
additional information | application note: in a gel without urea Tlp18.3 will run at 18 kDa, while in a gel with urea it runs at 25 kDa | |
selected references | Zienkiewicz et al. (2013).Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plan Physiol Biochem. March 16. |
application example
4, 2, and 1μg chl/lane (from left to right) of chloroplast prepared from Arabidopsis thaliana were separated on a 15% gel with 6M urea and blotted to PVDF. Filters were blocked (1h) with 5% milk powder and incubated with anti-Tlp18.3 antibody (1: 1000, 1h) followed by incubation with secondary HRP-coupled anti-rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL. Copyright(C) 1998-2025 生物器材網(wǎng) 電話:021-64166852;13621656896 E-mail:info@bio-equip.com
|