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                                當(dāng)前位置 > 產(chǎn)品目錄 > 試劑 > 植物試劑 > APX | L-ascorbate peroxidase
                                APX | L-ascorbate peroxidase
                                英文名稱:APX | L-ascorbate peroxidase總訪問(wèn):317
                                國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問(wèn):24
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS08368 最后更新:2024-12-5
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                                product information
                                background

                                APX plays a key role in plant antioxidant system by reducing hydrogen peroxide to water. Cellular localization includes chloroplast (tAPX and sAPX), cytosol (cAPX) and peroxisome (pAPX).

                                immunogen

                                synthetic peptide derived from Arabidopsis thaliana tAPX (acession At1g77490) and sAPX (At4g08390) protein sequences, coupled to BSA. Five out of twelve amino acids are also identical with cAPX1 (At1g07890), cPX2 (At3g09640) and pAPX (At4g35000)

                                antibody format

                                rabbit;

                                polyclonal;

                                serum;

                                lyophilized

                                quantity

                                200 µl

                                - for reconstitution add 200 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                Western blot (WB)

                                related products

                                AS06 180 | anti-cAPX

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1: 2000 with standard ECL (WB)

                                expected | apparent MW

                                25-38 kDa for A. thaliana

                                confirmed reactivity

                                Arabidopsis thaliana, Digitaria sanguinalis, Echinochla crus-galli, Liquidambar formosana, N. tabacum thylakoid-bound APX, stromal APX; Panicum milaceum, Schima superba, Silene vulgaris, Spinacia oleracea (stromal APX, thylakoid-bound)

                                predicted reactivity

                                O.sativa probable L-ascorbate peroxidase 6 chloroplastic; S. lycopersicum thylakoid-bound APX;

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information
                                selected references Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.

                                Kovácik et al. (2013). Oxidative stress, uptake and bioconversion of 5-fluorouracil in algae. Chemosphere. 2013 Dec 28. pii: S0045-6535(13)01679-2. doi: 10.1016/j.chemosphere.2013.11.074. (immunolocalization in algae)

                                Chen et al. (2013). Photosynthetic and antioxidant responses of Liquidambar formosana and Schima superba seedlings to sulfuric-rich and nitric-rich simulated acid rain. Plant Physiol & Biochem.

                                application example

                                                                                5 to 20 μg of total leaf protein from Arabidopsis thaliana (left panel) and chloroplast fractions (thylakoids and soluble, right panel) was separated on 15% polyacrylamide gel with 6M urea and blotted on PVDF. Filters were blocked 1h with 5% BSA, incubated with anti-APX antibody (1: 2000, 1h) followed by incubation with secondary HRP-coupled anti rabbit antibody (1: 10 000, 1h). Signal was detected with standard ECL. AS08 368 is reactive to thylakoid (tAPX, 38 kDa), stromal (sAPX, 33 kDa), peroxisomal (pAPX, 31 kDa) and cytoplasmic (cAPX1 + cAPX2, 25 kDa) forms of ascorbate peroxidases.
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