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                                L-cys | L-cysteine
                                英文名稱:L-cys | L-cysteine總訪問:343
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:5
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS08360 最后更新:2024-12-5
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                                product information
                                background

                                Cysteine (a non-essential amino acid) is a building block of antioxidant glutathione. With a thiol side chain, cysteine is an important structural and functional component of many proteins and enzymes.

                                immunogen

                                synthetic L-Cysteine conjugated to protein carrier (PC)

                                antibody format

                                rabbit;

                                polyclonal;

                                serum;

                                lyophilized

                                quantity

                                100 µl

                                - for reconstitution add 50 µl of sterile water and 50 µl of glycerol

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                immunocytochemistry (ICC)

                                related products

                                to be added when available

                                additional information

                                Compounds Cross-reactivity ratio (a)

                                L-Cysteine-G-(Pc) 1
                                DL-Homocysteine-G-(Pc) 1/10
                                L-Methionine-G-(Pc) 1/>1,000
                                L-Glutathion-G-(Pc) 1/>1,000
                                L-Cysteic acid-G-(Pc) 1/>1,000
                                L-Serine-G-(Pc) 1/>1,000
                                Free L-Cysteine 1/>10,000

                                (a) : L-Cysteine-G-(Pc) concentration/unconjugated or conjugated close-related compounds concentration at half displacement ; G =Glutaraldehyde. Cross-reactivity ratios were evaluated by competitive ELISA: a ratio of 1/1000 means that 1000 times higher concentration of immunogen is needed to switch off the specific signal by half.

                                application information
                                recommended dilution

                                1: 1000 - 1: 5 000 (IHC)

                                expected | apparent MW

                                n. a.

                                confirmed reactivity

                                L-cysteine

                                predicted reactivity

                                L-cysteine

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                example of immunohistochemical protocol can be found here.

                                Free cysteine will not attach to polystyrene ELISA plates. BSA-conjugated cysteine has to be used for this purpose.

                                selected references

                                Zechmann et al. (2006) Immunocytochemical localization of glutathione precursors in plant cells. J Electron Microsc (Tokyo), 55(3):173-81; Zechmann et al. (2007) Virus-induced changes in the subcellular distribution of glutathione precursors in Cucurbita pepo (L.). Plant Biol (Stuttg), 9(3):427-34


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