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Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3

英文名稱：	Toc75 \| Protein TOC75-3, chloroplastic, POTRA domain 3	總訪問：	249
國產/進口：	進口	半年訪問：	8
產地/品牌：	agrisera	產品類別：	植物試劑
規(guī) 格：	AS08351	最后更新：	2024-12-5
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產品介紹
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product information

background

OEP 75 or Toc75; Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446); In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87; Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane; YaeT; outer membrane protein assembly complex, (TIGR03303); FhaC; Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

immunogen

psTOC75; Predicted POTRA Domain #3; Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

antibody format

rabbit;

polyclonal;

serum;

lyophilized

quantity

200 µl

- for reconstitution add 200 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB), flow cytometry (FACS), immunolocalization (IL)

related products

AS08 345 | anti- Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1 rabbit antibodies

additional information

to be added when available

application information
recommended dilution		1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
expected \| apparent MW		88 \| 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
confirmed reactivity		Arabidopsis thaliana, Nicotiana tabacum, Pisum sativum, Spinacia oleracea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
predicted reactivity		Ricinus communis, Vitis vinifera, Populus trichocarpa
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction
selected references		to be added when available, antibody released February 2010

application example

580 ng of Chl of Pisum sativum plants (10 day old) (2, 4) and 10 µg of combined envelopes of Pisum sativum 10 day-old(1,3) were separated on 15% SDS-PAGE and blotted 2h to PVDF. PVDF was blocked 1h with 3% non-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Toc75 POTRA domain 3 antibodies AS08 351 (1:2000 and 1: 5000, 1h) and secondary donkey-anti-rabbit (1:20000, 1 h) antibody (HRP conjugated) in TBS-T containing 3% non fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with HRP substrate Peroxide solution & luminol detection reagent according to the manufacturers instructions (Millipore). Exposure time was 600 seconds.

Ashita Dave and Barry D. Bruce (UTK)

bio-equip.com

北京啟維益成科技有限公司（Beijing QiWei YiCheng Tech.,Ltd.(Qwbio)）成立于2005年12月，由從事生物領域多年銷售經(jīng)驗人士創(chuàng)立。目前我公司已經(jīng)與幾十家著名的試劑供應商建立了長期穩(wěn)定的合作關系，已成為眾多國際知名品牌的中國總代理或一級代理商；并且為滿足國內客戶的最新需求，我們也與時俱進地不斷與世界上更多的知名品牌洽談合作代理事宜；企業(yè)的主要目標是把國外有特點的生物前沿產品引進中國，讓國內科學研究機構和制藥企業(yè)盡早使用上最新型的科研產品。

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Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 3