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                                                              DnaK2 | heat shock protein 70-2 (HSP70-2)
                                                              英文名稱:DnaK2 | heat shock protein 70-2 (HSP70-2)總訪問:222
                                                              國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:6
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS08350 最后更新:2024-12-5
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                                                              product information
                                                              background

                                                              DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.

                                                              immunogen

                                                              full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence P22358

                                                              antibody format

                                                              rabbit

                                                              polyclonal

                                                              serum,

                                                              lyophilized

                                                              quantity

                                                              200 µl

                                                              storage

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                                              tested applications

                                                              western blot (WB)

                                                              related products

                                                              AS07 270 | anti-DnaK rabbit antibody

                                                              additional information

                                                              to be added when available

                                                              application information
                                                              recommended dilution

                                                              1: 1000 (WB)

                                                              expected | apparent MW

                                                              67.6 | 76 kDa

                                                              confirmed reactivity

                                                              Synechocystis PCC 6803

                                                              predicted reactivity

                                                              Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420, Nodularia spumigena CCY 9414, Nostoc sp., Synechococcus sp. PCC 7942 and other cyanobacterial strains

                                                              not reactive in

                                                              no confirmed exceptions from predicted reactivity known in the moment

                                                              additional information

                                                              not available at the moment

                                                              selected references

                                                              not available yet, antibody relased in February 2009


                                                              application example

                                                              10 µg of total protein from total cell extract of Synechocystis PCC 6803 wilde type (WT) and mutant strain that lacks Hsp16.6 were separated on 8 % gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1:1000 anti-DnaK2 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with standard ECL plus (GE Healthcare), exposure time 10 seconds.

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