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                                DnaK2 | heat shock protein 70-2 (HSP70-2)
                                英文名稱:DnaK2 | heat shock protein 70-2 (HSP70-2)總訪問:222
                                國產(chǎn)/進(jìn)口:進(jìn)口半年訪問:6
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS08350 最后更新:2024-12-5
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                                product information
                                background

                                DnaK2 belongs to the family of heat shock proteins 70 and functions as a chaperone involved in stress response. Dnak2 is heat induced but also present at high level under normal growth conditions.

                                immunogen

                                full length recombinant DnaK2 protein derived from Synechocystis PCC 6803 DnaK2 protein sequence P22358

                                antibody format

                                rabbit

                                polyclonal

                                serum,

                                lyophilized

                                quantity

                                200 µl

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS07 270 | anti-DnaK rabbit antibody

                                additional information

                                to be added when available

                                application information
                                recommended dilution

                                1: 1000 (WB)

                                expected | apparent MW

                                67.6 | 76 kDa

                                confirmed reactivity

                                Synechocystis PCC 6803

                                predicted reactivity

                                Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420, Nodularia spumigena CCY 9414, Nostoc sp., Synechococcus sp. PCC 7942 and other cyanobacterial strains

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                not available at the moment

                                selected references

                                not available yet, antibody relased in February 2009


                                application example

                                10 µg of total protein from total cell extract of Synechocystis PCC 6803 wilde type (WT) and mutant strain that lacks Hsp16.6 were separated on 8 % gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1:1000 anti-DnaK2 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with standard ECL plus (GE Healthcare), exposure time 10 seconds.

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