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application information | ||
recommended dilution | 1: 1000 (WB) | |
expected | apparent MW | 67.6 | 76 kDa | |
confirmed reactivity | Synechocystis PCC 6803 | |
predicted reactivity | Anabaena sp., Crocosphaera watsonii, Cyanothece sp. PCC 7424, Lyngbya sp. PCC 8106, Microcoleus chthonoplastes PCC 7420, Nodularia spumigena CCY 9414, Nostoc sp., Synechococcus sp. PCC 7942 and other cyanobacterial strains | |
not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
additional information | not available at the moment | |
selected references | not available yet, antibody relased in February 2009 |
application example 10 µg of total protein from total cell extract of Synechocystis PCC 6803 wilde type (WT) and mutant strain that lacks Hsp16.6 were separated on 8 % gel and blotted on nitrocellulose membrane. Filters were blocked (1h), incubated with 1:1000 anti-DnaK2 antibodies (2h) followed by incubation with 1: 2500 secondary anti-rabbit (1h) coupled to HRP and visualization with standard ECL plus (GE Healthcare), exposure time 10 seconds. | ![]() |