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                                eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2
                                英文名稱:eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 1B-alpha 2總訪問:467
                                國產(chǎn)/進口:進口半年訪問:7
                                產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                規(guī)       格:AS10678 最后更新:2024-12-5
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                                product information
                                background

                                eEF1B-alpha1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. This protein is a component of elongation factor B-complex which is composed of 2 elongation B-alpha-(1 and 2), 2 elongation factors B-beta (1 and 2) and 2 elongation factors B-gamma (1 and 2). Alternative names:

                                immunogen

                                recombinant eEF1B-alpha1 protein from Arabidopsis thaliana with no affinity tag, Q84WM9, At5g12110

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                100 µl

                                for reconstitution add 100 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha

                                AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

                                AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

                                AS07 265 | eEF1b | elongation factor eEF1b-beta protein

                                AS10 676 | eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2collection of antibodies to DNA/RNA metabolism
                                additional information

                                Antibody is recognizing elongation factor alpha 1 and alpha 2.

                                application information
                                recommended dilution

                                1: 3000 with standard ECL (WB)

                                expected | apparent MW

                                24 | 34 kDa

                                confirmed reactivity

                                Arabidopsis thaliana

                                predicted reactivity

                                dicots including: Glycine max, Solanum tuberosum, Vitis vinifera, monocots including: Oryza sativa, Zea mays

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                to be added when available

                                selected references

                                to be added when available, antibody released in June 2010


                                application example
                                western blotdetection using anti-elongation factor alpha 1 and 2 antibodies

                                10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins alpha 1 (3) and alpha 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Amersham diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds. The primary antibody could be reused if it is kept at 4ºC for 2 weeks and if frozen at -20ºC for long time. The pre-immune did not cross react with any plant protein non-specifically. Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

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