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                                eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2
                                英文名稱:eEF1B-gamma1 and 2 | elongation factor 1-gamma 1 and 2總訪問:378
                                國產/進口:進口半年訪問:21
                                產地/品牌:agrisera產品類別:植物試劑
                                規(guī)       格:AS10676 最后更新:2024-12-5
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                                product information
                                background

                                eEF1B-gamma1 protein belongs to a family of elongation factors, proteins which are involved in translational elongation. Alternative names: EF-1-gamma 1, eEF-1B gamma 1

                                immunogen

                                recombinant eEF1B-gamma1 protein from Arabidopsis thaliana with no affinity tag, O04487, At1g09640

                                antibody format

                                rabbit

                                polyclonal

                                serum

                                lyophilized

                                quantity

                                200 µl

                                for reconstitution add 200 µl of sterile water

                                storage

                                store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

                                tested applications

                                western blot (WB)

                                related products

                                AS11 1633 | EF1A | elongation factor 1-alpha / EF-1-alpha

                                AS10 679 | eEF1B-alpha2 | elongation factor 1B-alpha 2

                                AS10 678 | eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2

                                AS10 677 | eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2

                                AS07 265 | eEF1b | elongation factor eEF1b-beta protein

                                collection of antibodies to DNA/RNA metabolism

                                additional information

                                antibody is recognizing elongation factor 1-gamma 1 and gamma 2

                                application information
                                recommended dilution

                                1: 3000 with standard ECL (WB)

                                expected | apparent MW

                                46 kDa

                                confirmed reactivity

                                Arabidopsis thaliana

                                predicted reactivity

                                dicots including: Solanum tuberosum, Vitis vinifera, monocots including: Oryza sativa, Zea mays

                                not reactive in

                                no confirmed exceptions from predicted reactivity known in the moment

                                additional information

                                to be added when available

                                selected references

                                to be added when available, antibody released in June 2010


                                application example
                                Western blot detection using anti-elongation factor gamma 1 and 2antibodies

                                10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22ºC (1) or heat treated at 38ºC for 2 hours prior to protein extraction (2). As positive control 10 ng of recombinant elongation factor proteins gamma 1 (3) and gamma 2 (4) were separated side by side with the plant samples on 11% SDS-PAGE and blotted to nitrocellulose (Bio-rad). Blots were blocked following transfer with 5% low fat milk in low salt buffer for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 2000 for 2h at room temperature with agitation in the blocking solution. The primary antibody solution was removed and the blot was rinsed briefly twice, then washed 4 times for 15 min each at room temperature with agitation using low salt buffer. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxides conjugated, from Amersham diluted to 1:2500 for 1h at room temperature with agitation then washed as above and treated with ECL detection reagent according to the manufacturers instructions. Exposure time was 5 seconds.

                                Low salt buffer components are 10 mM Tris (pH 7.6), 68 mM NaCl and 0.05 % Triton X-100.

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