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application information | ||
recommended dilution | 1: 1000 with standard ECL (WB) | |
expected | apparent MW | 17.7 kDa | |
confirmed reactivity | Arabidopsis thaliana, Agave tequiliana var. Weber, Cucumis sativus, Pinellia ternata, Silene vulgaris | |
predicted reactivity | higher plants | |
not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
additional information | to be added when available | |
selected references | Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print. Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803. |
application example 15 µg of total protein from (HS) heat shocked (38ºC/2h) Arabidopsis thaliana , (C) Arabidopsis thaliana control plants, (1,2,5,10) 1,2,5,10 ng of recombinant puridfied HSP17.7 were separated on 15% SDS-PAGE and blotted 1h to nitrocellulose(Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam). Development was done ECL (Thermo Scientific) according to the recommendations of the manufacturers. | ![]() |