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                                                              HSP90 | Heat shock protein 90
                                                              英文名稱:HSP90 | Heat shock protein 90總訪問:1423
                                                              國(guó)產(chǎn)/進(jìn)口:進(jìn)口半年訪問:25
                                                              產(chǎn)地/品牌:agrisera產(chǎn)品類別:植物試劑
                                                              規(guī)       格:AS05063 最后更新:2024-12-5
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                                                              product information




                                                              background
                                                               

                                                              HSP90 is a ubiquitous chaperone involved in numerous cellular processes. Members of this family of proteins play important roles in allowing a selected group of intracellular signaling molecules reach and maintain functionally active conformations.Alternative names: for hsp90 alpha: HSP 86, renal carcinoma antigen NY-REN-38, for hsp90 beta: hsp84




                                                              immunogen
                                                               

                                                              KLH-conjugated synthetic peptide chosen from a highly conserved region of hsp90 found in both the alpha P07900 and beta P08238 form of the protein. The target peptide is perfectly conserved in animals.




                                                              antibody format
                                                               



                                                              rabbit;


                                                              polyclonal;



                                                              serum;


                                                              lyophilized







                                                              quantity
                                                               



                                                              100 µl


                                                              - for reconstitution add 100 µl of sterile water







                                                              storage
                                                               

                                                              store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.




                                                              tested applications
                                                               

                                                              Western blot (WB)




                                                              related products
                                                               

                                                              AS05 061 | anti-salmonid HSP70

                                                              AS05 061A | anti-salmonid HSP70, suitable for immunoprecipitation

                                                              AS05 062 | anti-salmonid HSC70

                                                              AS05 083 | anti-HSP70/HSC70 global antibody

                                                              AS05 083A | anti-HSP70/HSC70 global antibody, affinity purified




                                                              additional information
                                                               

                                                              to be added when available






                                                              application information




                                                              recommended dilution
                                                               

                                                              1: 5 000 with standard ECL (WB)




                                                              expected | apparent MW
                                                               

                                                              84-86 kDa




                                                              confirmed reactivity
                                                               

                                                              salmon, human




                                                              predicted reactivity
                                                               

                                                              fishes, hen, mammals




                                                              not reactive in
                                                               

                                                              no confirmed exceptions from predicted reactivity known in the moment




                                                              additional information
                                                               

                                                              in salmonid fish a cross-reactive band at approximately 40 kDa is observed; antibody will also detect a human recombinant HSP90 protein




                                                              selected references
                                                               

                                                              LeBlanc et al. (2011). Chronic social stress impairs thermal tolerance in the rainbow trout (Oncorhynchus mykiss). J Exp Biol. 15;214:1721-31.

                                                              Rendell et al. (2006) Development-dependent differences in intracellular localization of stress proteins (hsps) in rainbow trout, Oncorhynchus mykiss, following heat shock. Comparative Biochemistry and Physiology, Part D; 1(2):238 – 252. Osborne et al. (2007) The role of hsp90 in 17alpha-ethynylestradiol-induced endocrine disruption in rainbow trout hepatocytes. Ecotoxicol Environ Saf. 68(1):13-9.






                                                              application example - Western blot:

                                                               




                                                              ||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menu bar or contact us at  [email protected]









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