product information

background
 

HSP90 is a ubiquitous chaperone involved in numerous cellular processes. Members of this family of proteins play important roles in allowing a selected group of intracellular signaling molecules reach and maintain functionally active conformations.Alternative names: for hsp90 alpha: HSP 86, renal carcinoma antigen NY-REN-38, for hsp90 beta: hsp84

immunogen
 

KLH-conjugated synthetic peptide chosen from a highly conserved region of hsp90 found in both the alpha P07900 and beta P08238 form of the protein. The target peptide is perfectly conserved in animals.

antibody format
 

rabbit;

polyclonal;

serum;

lyophilized

quantity
 

100 µl

- for reconstitution add 100 µl of sterile water

storage
 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB)

related products
 

AS05 061 | anti-salmonid HSP70

AS05 061A | anti-salmonid HSP70, suitable for immunoprecipitation

AS05 062 | anti-salmonid HSC70

AS05 083 | anti-HSP70/HSC70 global antibody

AS05 083A | anti-HSP70/HSC70 global antibody, affinity purified

additional information
 

to be added when available

application information

recommended dilution
 

1: 5 000 with standard ECL (WB)

expected | apparent MW
 

84-86 kDa

confirmed reactivity
 

salmon, human

predicted reactivity
 

fishes, hen, mammals

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

in salmonid fish a cross-reactive band at approximately 40 kDa is observed; antibody will also detect a human recombinant HSP90 protein

selected references
 

LeBlanc et al. (2011). Chronic social stress impairs thermal tolerance in the rainbow trout (Oncorhynchus mykiss). J Exp Biol. 15;214:1721-31.

Rendell et al. (2006) Development-dependent differences in intracellular localization of stress proteins (hsps) in rainbow trout, Oncorhynchus mykiss, following heat shock. Comparative Biochemistry and Physiology, Part D; 1(2):238 – 252. Osborne et al. (2007) The role of hsp90 in 17alpha-ethynylestradiol-induced endocrine disruption in rainbow trout hepatocytes. Ecotoxicol Environ Saf. 68(1):13-9.

application example - Western blot:

 

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