product information

background
 

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids and thyroid hormones, and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

immunogen
 

full length native protein P02769

antibody format
 

hen;

polyclonal;

affinity purified IgY at 1 mg/ml in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2;

liquid

quantity
 

200 µl (1 µg/µl)

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB), immunodifusion (ID)

related products
 

AS99 002 | anti-BSA rabbit antibody

AS02 023 | anti-BSA hen antibody

IMS06-035-327 | anti-BSA hen antibody, biotinylated

additional information
 

affinity purified IgY on bovine serum albumin agarose

 

 

application information

recommended dilution
 

1: 5 000 with standard ECL (WB)

expected | apparent MW
 

67 kDa

confirmed reactivity
 

bovine

predicted reactivity
 

goat, sheep

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

only one precipitin arc in immunodiffusion against normal bovine serum, dilution 1:100, or bovine albumin. Only one precipitin arc in immunoelectrophoresis against normal bovine serum or bovine serum albumin.

selected references
 

to be added when available

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