product information

background
 

Complement component C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa; beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600 mg/L. It is a protein of the classical complement pathway. When activated by C1s, peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

immunogen
 

full lenth native protein P0C0L4 purified from human plasma

antibody format
 

hen;

polyclonal;

total IgY fraction at 25.9 mg/ml in 0.9% NaCl, 0.1% sodium azide

liquid

quantity
 

200 µl

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB)

related products
 

IMS02-032-303 | anti-Complement component C4

IMS06-032-304 | anti-Complement component C4, biotinylated

IMS01-066-309 | anti-Complement factor H

additional information
 

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

application information

recommended dilution
 

use at an assay dependent dilution (WB)

expected | apparent MW
 

35 kDa

confirmed reactivity
 

human

predicted reactivity
 

human

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

Only one precipitin arcs in immunodiffusion against normal human serum or human C4. Reacts with α and β chain in electroblotting.

selected references
 

to be added when available

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