product information

background
 

Tumor Necrosis Factor alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

immunogen
 

purified full length recombinant protein P01375

antibody format
 

hen;

polyclonal;

affinity purified IgY in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1 % sodium azide, pH 7.2;

liquid

quantity
 

200 µl

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB), ELISA (ELISA)

related products
 

IMS01-142-310 | Etanercept (Enbrel^®), recombinant human tumor necrosis factor receptor

additional information
 

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation; labelled with biotin.

Total IgY concentration for this antibody is 15 mg/ml

application information

recommended dilution
 

1: 5000 with standard ECL (WB)

expected | apparent MW
 

26 kDa

confirmed reactivity
 

human

predicted reactivity
 

cat, dog, horse, pig, primates

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

the antibody, in dilution 1:5 000, will detect 10 ng of SDS denatured and reduced human TNFa on western blots

selected references
 

to be added when available

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