product information

background
 

Basic Fibroblast Growth Factor (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth.  FGF2  mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

immunogen
 

purified full length native protein P09038

antibody format
 

hen;

polyclonal;

total IgY fraction at 13 mg/ml in 0.9 % NaCl, 0.1 % sodium azide;

liquid

quantity
 

200 µl

storage
 

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

tested applications
 

Western blot (WB)

related products
 

to be added when available

additional information
 

the IgY fraction is isolated by a two-step PEG precipitation procedure followed by ammonium sulphate precipitation

application information

recommended dilution
 

1: 1000 with standard ECL (WB), 1: 1000 (ELISA)

expected | apparent MW
 

18 kDa

confirmed reactivity
 

human

predicted reactivity
 

bovine, chicken, mouse, rat, sheep

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

reacts with  human recombinant FGF2. No reactivity with other human serum proteins

selected references
 

to be added when available

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