product information

background
 

3-hydroxybutyrate dehydrogenase (EC=1.1.1.30) is an oxidoreductase which participates in synthesis and degradation of ketone bodies and butanoate metabolism. Alternative names: NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase,  -D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase.

immunogen
 

3-hydroxybutyrate dexydrogenase isolated and purified from Rhodobacter sphaeroides

antibody format
 

rabbit

polyclonal

purified IgG in phosphate buffer saline,

lyophilized

quantity
 

10 mg

for reconstitution please add 1 ml of sterile destilled water.

storage
 

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications
 

western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)

related products
 

AS09 563 | anti-3-hydroxybutyrate dexydrogenase (biotinylated)

AS09 564 | anti-3-hydroxybutyrate dexydrogenase (affinity purified)

additional information
 

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

application information

recommended dilution
 

1: 100 - 1: 500 for techniques listed above

expected | apparent MW
 

26.9 kDa

confirmed reactivity
 

Rhodopacter shpaeroides

predicted reactivity
 

Sagittula stellata, Silicibacter sp.

not reactive in
 

no confirmed exceptions from predicted reactivity known in the moment

additional information
 

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

selected references
 

to be added when available

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