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product information | |||||||
| background |
Helicobacter pylori is nowadays recognised as one of the most common human pathogens, and has been recently defined as a class 1 carcinogen. It has an ability to colonise gastric mucosa, where it causes chronic gastritis and is a major cause for duodenal and sometimes gastric ulcers. | ||||||
| immunogen |
whole cells of Helicobacter pylori, standard laboratory strain (17-1 e.g. CCUG 17875) emulsified with an adjuvant. | ||||||
| antibody format |
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| quantity |
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| storage |
store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes. | ||||||
| tested applications |
western blot (WB) ELISA (ELISA), immunolocalization (IL) | ||||||
| related products |
AS10 1346 | Anti-Helicobacter pylori, rabbit antibody AS10 1373 | Anti-Helicobacter pylori, goat antibody | ||||||
| additional information |
IgY concentration is 23.5 mg/ml | ||||||
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application information | ||
| recommended dilution |
1: 5000 to 1:20 000 with ECL system (WB) 1:10 000 to 1:20 000 (ELISA), 1: 500 (IL) | |
| expected | apparent MW |
n.a. | |
| confirmed reactivity |
Helicobater pylori | |
| predicted reactivity |
n.a. | |
| not reactive in |
no confirmed exceptions from predicted reactivity known in the moment | |
| additional information |
Western blot has been done using whole extracts of Helicobacter pylori. Antibody recognised several low and high molecular weight H. pylori proteins. Some of them are from the surface of bacteria. | |
| selected references |
not available at the moment | |
application example
Panel 1: secondary antibody only
A. - Hoechst, B. - anti- chicken Alexa 568, dilution 1: 500, C. - Hoechst and anti-chicken Alexa 568
Panel 2: primary and secondary antibody detection
A. - Hoechst, B. - chicken anti-Helicobacter pylori at 1: 200 and anti-chicken Alexa 568, dilution 1: 500, C. - Hoechst, chicken anti-Helicobacter pylori at 1: 200 and anti-chicken Alexa 568
Protocol Immunofluorescence for Helicobacter pylori
Harvest Helicobacter pylori from blood agar plates in 0.9% NaCl
Put onto coverslips in 24-well plate and spin down at 1500g for 5min
Fix with 4% Paraformaldehyde in PBS
Wash with PBS
5 min triton X-100 0.2% in PBS
Wash with PBS
Block with 4% foetal calf serum in PBS
Chicken anti-Helicobacter primary antibody (Agrisera cat# AS01 020) diluted in 4% FSC in PBS 1 hour at room temp
Wash with PBS
Goat anti-Chicken Alexa fluor 568 (Invitrogen cat# A11041) in PBS 30 min at room temperature
Wash with PBS and water
3 min Hoechst 1ug/ml in water
Wash in water
Mount
