西寶生物科技（上海）股份有限公司

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Anti Iba1, Rabbit (for Immunocytochemistry)——西寶生物提供

抗Iba1，兔源（免疫細胞化學用）

鈣離子作為信號轉導通道中的第一信使，在所有細胞包括中樞神經(jīng)細胞的生命活動中起重要作用。鈣離子與不同的鈣結合蛋白結合后進行級聯(lián)反應。Iba1是能與巨噬細胞和小膠質細胞發(fā)生特異性結合的鈣結合蛋白，當這些細胞激活后，Iba1的表達量上調，因此Iba1通常作為鑒定小膠質細胞的標記物使用。近來研究發(fā)現(xiàn)，小膠質細胞除了提供營養(yǎng)、保護神經(jīng)的作用外，還被證明可產生NO、TNF-a、IL-1β等物質，因此將小膠質細胞定義為中樞神經(jīng)系統(tǒng)的巨噬細胞樣吞噬細胞，具有重要的免疫細胞作用。當病原菌、病毒入侵中樞神經(jīng)系統(tǒng)時會起免疫監(jiān)御的作用。近來研究發(fā)現(xiàn)，該細胞可能與神經(jīng)退行性疾病的發(fā)生和發(fā)展有關。本產品是可與小膠質細胞發(fā)生特異性反應的兔多抗，配合對星形膠質細胞有特異性的GFAP單抗，可進行雙染色。

抗原：合成肽（Iba1 的C末端序列）

外觀：TBS溶液（1mg/ml）

提純：抗原親和提純

特異性：對小膠質細胞、巨噬細胞特異反應。不會與神經(jīng)元、星形膠質細胞發(fā)生交叉反應。能與人、小鼠、大鼠Iba1反應。

用法：IBA1抗體，兔源（免疫細胞化學用）適合免疫細胞化學。只需1~2μg/ml即可進行實驗。

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英文參考：

Summary

This product is for research use only. Do not administer it to human.

Antibodies against Macrophage/Microglia-specific Protein Iba1

Calcium ions are known to be one of the most important signal mediators in all cells including central nervous system (CNS) cells. Calcium ions exert their signaling activity through association with various calcium binding proteins, many of which are classified into a large protein family, the EF hand protein family. Iba1 is a 17-kDa EF hand protein that is specifically expressed in macrophages/ microglia and is upregulated during the activation of these cells.Wako has launched rabbit polyclonal antibodies were raised against a synthetic peptide corresponding to the Iba1 carboxy-terminal sequence, which was conserved among human, rat and mouse Iba1 protein sequences. These antibodies are specifically reactive to microglia/ macrophages, are appropriate for immuno-double staining of brain tissues and cell culture in combination with monoclonal antibody to GFAP, which specifically reacts to astrocyte.

Rabbit Anti Iba1 antibody is raised a synthetic peptide corresponding to C-terminus of Iba1.

Preparation: Purified by the antigen affinity chromatography from rabbit antisera and prepared in TBS solution. Contains no preservatives and stabilizers.

Specificity: Specific to microglia and macrophage, but not cross-reactive with neuron and astrocyte. Reactive with human, mouse and rat Iba1.

Working Concentration: Immunocytochemistry 1-2 micro-g/mL

Storage:Store at -20 degrees C. After opening, aliquot contents and freeze at -20 degrees C.

Package Size: 50 ug (100 uL) (ug: micro-g; uL: micro-L)

References:

Imai, Y., Ibata, I., Ito, D., Ohsawa, K. and Kohsaka, S.: Biochem. Biophys. Res. Commun., 224, 855 (1996).

Ito, D., Imai, Y., Ohsawa, K, Nakajima, K, Fukuuchi, Y. and Kohsaka, S.: Brain Res. Mol. Brain Res., 57, 1 (1998).

Ohsawa, K., Imai, Y., Kanazawa, H., Sasaki, Y. and Kohsaka, S.: J. Cell Sci., 113, 3073 (2000).

Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S. and Imai, Y.: Biochem. Biophys. Res. Commun., 286, 292 (2001).

Kanazawa, H., Ohsawa, K., Sasaki, Y., Kohsaka, S. and Imai, Y.: J. Biol. Chem., 277, 20026(2002).

Wako catalog #016-20001 Anti Iba1 polyclonal antibody, Rabbit, for Western Blotting (50 micrograms (100 microlitters))Working Concentration: Westernblot 0.5 - 1 micrograms/mL 

[Recommended preparations]

Frozen mouse brain tissue or cultured cells should be perfusion-fixed with 4% paraformaldehyde-PBS. Then the tissue section is prepared. After those preparation, the tissue section and cultured cells are immunostained with anti Iba1 antibody.